Immune cells rely on receptor to signal counterattack on parasitic worms

HERSHEY, Pa. — Immune cells, called macrophages, may rely on the compound interleukin 4 to signal an attack to counter infection by parasitic worms, according to an international team of researchers, including Zissis C. Chroneos, associate professor of pediatrics, and microbiology and immunology at Penn State College of Medicine.

The findings could lead to better drugs to fight common infections.

It is estimated that parasitic worms infect hundreds of millions of people, mainly in the developing world. These roundworms and flatworms — collectively known as helminths — often infect the intestinal system, causing anemia and malnutrition.

But worms can also infect other organs and systems, such as the lungs, urinary tract or bloodstream, causing a range of health problems. For example, the common worm infection schistosomiasis can lead to high blood pressure in lung arteries.

Chroneos and his co-investigators studied a roundworm, called Nippostrongylus brasiliensis, that infects the lungs of rodents. They used a compound called interleukin 4, or IL-4, to activate large white blood cells -- macrophages -- to kill the parasite and promote healing in the lung tissue.

The study, published in Science, helps to explain how this process works. The researchers discovered that IL-4 helps the outer layer of lung cells to boost production of the defense protein SP-A. This protein binds macrophages and enhances their ability to multiply and activate against the parasite.

Chroneos discovered that the SP-A protein binds at a receptor called myosin 18A. IL-4 triggers the appearance, or expression, of this receptor on macrophages.

"You cannot kill the worm if you don't have myosin 18A," Chroneos said.

To test this, researchers in his lab developed antibodies to block the myosin 18A receptor. When it was blocked in mice, rats and human lung cells, the growth of macrophages against parasites was reduced.

The findings have implications beyond the lungs, Chroneos said.

The researchers found that the receptor myosin 18 also allows macrophages to bind to a different defense protein called C1q in the abdomen of mice. Macrophages from mouse liver, spleen and fat tissue expressed myosin 18A when exposed to the immune compound IL-4, which suggests that the receptor is important for fighting infections throughout the body.

In the future, the new knowledge could be used to develop drugs that work against infections by triggering the IL-4 immune response.

"Now that we know how this works, we can potentially use molecules that activate the myosin 18A pathway or the IL-4 pathway to kill the disease," Chroneos said.

Other researchers on this project were Carlos M. Minutti, from the Complutense University of Madrid, the Instituto de Salud Carlos III, and the University of Edinburgh; Lucy H. Jackson-Jones, Johanna A. Knipper, Nicola Logan, Emma Rinqvist, David A. Ferenbach and Dietmar M. Zaiss, from the University of Edinburgh; Belén García-Fojeda and Cristina Casals from the Complutense University of Madrid and the Instituto de Salud Carlos III; Tara E. Sutherland from the University of Edinburgh and the University of Manchester; Raquel Guillamat-Prats and Antonio Artigas from the Instituto de Salud Carlos III and the Universitat Autònoma de Barcelona; Cordula Stamme from the University of Lübeck; and Judith E. Allen from the University of Edinburgh and the University of Manchester.

Minutti was recipient of fellowships from the Spanish Ministry of Science and Institute of Health Carlos III. This work was supported by the Spanish Ministry of Economy and Competitiveness, Institute of Health Carlos III, Medical Research Council UK, Wellcome Centre for Cell-Matrix Research, Medical Research Council, European Union, and National Institute of Health.

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Last Updated May 16, 2017